Tryptic digest of BSA using Kinetex 2.6u C18 150 x 4.6mm ID

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% TFA

B: Acetonitrile with 0.1% TFA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

30

50

50

Flow Rate

1.20 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info