Hu IgG2 k Lys-C & Asp-N Digest on Kinetex C18 2.6u

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA and 2% Acetonitrile in Water

B: 0.085% TFA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

2

98

2

2

35

52

48

3

40

30

70

Flow Rate

1.00 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (25°C)

Detection Info