USP 621: Allowable changes Column Diameter-3.0mm ID
LC Conditions
Column
Luna 5 µm C18(2) 100 Å, LC Column 150 x 3 mm, Ea
Brand
Luna
Part No
00F-4252-Y0
Phase Name
C18(2)
Elution Type
Isocratic
Mobile Phase
A: See analyst note
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
100
0
Flow Rate
0.36 mL/min
Temperature
22°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Sample Notes
Isocratic: 100% of 2.0g of Sodium Octanesulphonate + 0.8g of Tetrabutyl Ammonium Hydrogen Sulphate in 2L of 360ml Methanol + 40ml of THF+1600ml of 3.4g of potassium Dihydrogen Phosphate in 2L of water (pH adjusted to 3.0 using Phosphoric acid)
Order Items Used in This Application
- 1. Atenolol
- 2. Impurity B
- 3. Impurity F
- 4. Impurity G
- 5. Impurity I
- 6. Impurity J
-OEChem-06211003142D<br><br> 41 41 0 1 0 0 0 0 0999 V2000<br> 5.4641 -0.6550 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 -2.6550 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1962 3.3450 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -3.6550 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3301 4.8450 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -2.6550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 -2.1550 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 2.8660 -4.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 -1.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -5.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -3.6550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 0.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 2.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3301 0.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 0.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3301 1.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 1.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 3.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3301 3.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.5200 -2.0724 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1215 -2.7627 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 -3.0050 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4030 -4.4650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2690 -3.9650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3860 -0.5724 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.9875 -1.2627 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2460 -5.1550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -5.7750 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4860 -5.1550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3100 -3.1181 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4631 -3.3450 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.6900 -4.1919 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 -3.2750 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8671 0.5350 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0611 0.5350 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8671 2.1550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.0611 2.1550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2520 3.9276 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8535 3.2373 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.8671 5.1550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7932 5.1550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 9 1 0 0 0 0<br> 1 12 1 0 0 0 0<br> 2 7 1 0 0 0 0<br> 2 33 1 0 0 0 0<br> 3 19 2 0 0 0 0<br> 4 6 1 0 0 0 0<br> 4 8 1 0 0 0 0<br> 4 24 1 0 0 0 0<br> 5 19 1 0 0 0 0<br> 5 40 1 0 0 0 0<br> 5 41 1 0 0 0 0<br> 6 7 1 0 0 0 0<br> 6 20 1 0 0 0 0<br> 6 21 1 0 0 0 0<br> 7 9 1 0 0 0 0<br> 7 22 1 0 0 0 0<br> 8 10 1 0 0 0 0<br> 8 11 1 0 0 0 0<br> 8 23 1 0 0 0 0<br> 9 25 1 0 0 0 0<br> 9 26 1 0 0 0 0<br> 10 27 1 0 0 0 0<br> 10 28 1 0 0 0 0<br> 10 29 1 0 0 0 0<br> 11 30 1 0 0 0 0<br> 11 31 1 0 0 0 0<br> 11 32 1 0 0 0 0<br> 12 14 2 0 0 0 0<br> 12 15 1 0 0 0 0<br> 13 16 2 0 0 0 0<br> 13 17 1 0 0 0 0<br> 13 18 1 0 0 0 0<br> 14 16 1 0 0 0 0<br> 14 34 1 0 0 0 0<br> 15 17 2 0 0 0 0<br> 15 35 1 0 0 0 0<br> 16 36 1 0 0 0 0<br> 17 37 1 0 0 0 0<br> 18 19 1 0 0 0 0<br> 18 38 1 0 0 0 0<br> 18 39 1 0 0 0 0<br>M END
Compound Name
Atenolol
CID
2249
Molecular Formula
C14H22N2O3
Molecular Weight
266
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3
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