Extraction of Indole 3 carboxylic acid from Strata-X-A on Gemini 5u, C18, 150x4.6mm Column

LC Conditions

Column

Gemini 5 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea

Brand

Gemini

Part No

00F-4435-E0

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: 20 mM KH2PO4(pH 2.5)

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X-A 33 µm Polymeric Strong Anion, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S123-TAK

Pretreatment Note

Elution solvent was applied in 2 aliquots of 500uL followed by evaporation to dryness at 45 deg C under N2 and reconstitute with 500 uL initial mobile phase (20mm KH2PO4(PH 2.5)/ ACN=75/25) spiked with 50ug/mL Salicylic acid (IS)

Condition 1

1 mL Methanol at 1 mL/min

Condition 2

1 mL Water at 1 mL/min

Load

Load 500.00 µL Sample diluted in Water

Wash 1

1 mL 50 mm Na acetate, PH 7.0 at 1 mL/min

Wash 2

1 mL Methanol at 1 mL/min

Dry

2 mins at 10 of Hg

Elution 1

1000 mL 5% FA/MeOH at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

500 µL 20mm KH2PO4(PH 2.5)/ ACN=75/25