Fluoroquinolones from Propolis using Strata-X and Gemini-NX by LCMS

LC Conditions

Column

Gemini 3 µm NX-C18 110 Å, LC Column 50 x 2 mm, Ea

Brand

Gemini

Part No

00B-4453-B0

Phase Name

NX-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

5

10

90

3

6

10

90

4

6

10

90

Flow Rate

0.40 mL/min

Temperature

40°C

Detection

Mass Spectrometer (MS) @ (550°C)

Detection Info

Sample Notes

Source temp: 450ºC Polarity: Postive Dwell: 25 msec

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 200 mg / 6 mL, Tubes, 30/Pk

Part No

8B-S100-FCH

Condition 1

3 mL Methanol at 2 mL/min

Condition 2

3 mL Water at 2 mL/min

Load

Load 10.00 mL Sample diluted in Phosphate buffer with 2% TCA

Wash 1

2 at 2

Dry

5min @ >10 in Hg

Elution 1

2 mL Methanol/ACN/Acetic acid 49/49/2 at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

500 µL 0.1% Formic acid