EPA 552.1 by Strata-X-A on a ZB-XLB-HT

GC Conditions

Official Method

EPA 552

Column

Zebron ZB-XLB-HT, GC Cap. Column 30 m x 0.32 mm x 0.25 µm, Ea

Part No

7HM-G024-11

Phase Name

ZB-XLB-HT

Injection

Pulsed @ 250°C

Carrier Gas

Constant Flow Helium, 2.3 mL/min

Detection

Electron Capture (ECD) @ (340°C)

Detection Info

Sample Notes

Derivatization: Add 1mL of MTBE to the eluent in a screw cap vial or test tube. Heat the sample at 50ºC for 1.5 hours. Remove and allow the sample to cool to room temperature. Slowly add 10mL of saturated sodium bicarbonate and invert the sample several times releasing carbon dioxide gas frequently. Aspirate 100µL of the organic layer and transfer to a 2mL vial with insert. Add 10µL of internal standard (1,2,3-Trichloropropane) and inject into GC-ECD system.

Sample Preparation Details

Pretreatment Note

Elute: 1mL of 10% Sulfuric acid in methanol, eluent should be collected in a screw cap vial or test tube to accommodate derivatization. Note: Blank sample extract, post spiked was processed as above to prepare an extracted ref sample (Eref) for absolute recovery calculation Derivatization: Add 2mL of MTBE to the eluent in a screw cap vial or test tube. Heat the sample at 50ºC for 1.5 hours. Remove and allow the sample to cool to room temperature. Slowly add 10mL of saturated sodium bicarbonate and invert the sample several times releasing carbon dioxide gas frequently. Aspirate 100µL of the organic layer and transfer to a 2mL vial with insert. Add 10µL of internal standard (1, 2, 3-Trichloropropane) and inject into GC-ECD system.