EPA 552.1 by Strata-X-A on a ZB-MR2

GC Conditions

Official Method

EPA 552

Column

Zebron ZB-MultiResidue-2, GC Cap. Column 30 m x 0.32 mm x 0.25 µm, Ea

Part No

7HM-G017-11

Phase Name

ZB-MultiResidue-2

Injection

Pulsed @ 250°C

Carrier Gas

Constant Flow Helium, 2.3 mL/min

Detection

Electron Capture (ECD) @ (340°C)

Detection Info

Sample Notes

Derivatization: Add 1mL of MTBE to the eluent in a screw cap vial or test tube. Heat the sample at 50ºC for 1.5 hours. Remove and allow the sample to cool to room temperature. Slowly add 10mL of saturated sodium bicarbonate and invert the sample several times releasing carbon dioxide gas frequently. Aspirate 100µL of the organic layer and transfer to a 2mL vial with insert. Add 10µL of internal standard (1,2,3-Trichloropropane) and inject into GC-ECD system.

Sample Preparation Details

Condition 1

2 mL Methanol at 1 mL/min

Condition 2

2 mL Water at 1 mL/min

Load

Load 100.00 mL Sample diluted in Water

Wash 1

1 mL Methanol at 1 mL/min

Dry

Do not let sorbent bed go dry

Elution 1

1 mL 10% sulfuric acid in methanol at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

0 µL initial mobile phase