Insulin impurity profile using Kinetex 2.6µm C18 150x4.6mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand

Kinetex

Part No

00F-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 20mM Potassium phosphate pH 2.7

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

76

24

2

30

10

90

Flow Rate

1.20 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info