Bivalirudin Purification-Fraction 33.2 min

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Kinetex

Part No

00D-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in Water

B: 0.1% TFA in ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

77

23

2

8

69

31

3

8.1

77

23

4

11

77

23

Flow Rate

1.85 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info