Iso-alpha acids using Kinetex C18 2.6µm 50x4.6mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Kinetex

Part No

00B-4462-E0

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: Methanol

B: Water

C: Phosphoric acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

24

Flow Rate

1.40 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info