Iso-alpha acids extracted from beer by Strata-X using Kinetex C18 2.6µm 100x4.6 mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Kinetex

Part No

00D-4462-E0

Phase Name

C18

Elution Type

Isocratic

Mobile Phase

A: Methanol

B: Water

C: Phosphoric acid

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

24

Flow Rate

1.40 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 200 mg / 6 mL, Tubes, 30/Pk

Part No

8B-S100-FCH

Condition 1

4 mL Acidified methanol (0.05% H3PO4) at 1 mL/min

Condition 2

4 mL Water at 1 mL/min

Load

Load 5.00 mL Sample diluted in Red Stripe Beer

Wash 1

4 mL 40% methanol in water at 1 mL/min

Dry

5 min @ >10 inches of Hg

Elution 1

2 mL Acidified methanol at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

500 µL Mobile phase