USP Hydrocortisone Acetate on Kinetex 2.6µm XB-C18 75x4.6mm

LC Conditions

Column

Kinetex 2.6 µm XB-C18 100 Å, LC Column 75 x 4.6 mm, Ea

Brand

Kinetex

Part No

00C-4496-E0

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: 80:20 Water:Acetonitrile

B: 70:30 Acetonitrile:Water

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

5

90

10

3

25

10

90

4

30

10

90

5

35

90

10

6

40

90

10

Flow Rate

1.00 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info