ABN16 (acids, bases, neutrals) Mix using Kinetex 2.6µm XB-C18, 50x2.1mm

LC Conditions

Column

Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4496-AN

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% formic acid/water

B: 0.1% formic acid/ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

0.2

95

5

3

4.2

5

95

4

4.21

95

5

5

5.5

95

5

Flow Rate

0.80 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info