USP Loratadine with two related compounds (A&B) on Kinetex 2.6µm XB-C18 75x4.6mm

LC Conditions

Column

Kinetex 2.6 µm XB-C18 100 Å, LC Column 75 x 4.6 mm, Ea

Brand

Kinetex

Part No

00C-4496-E0

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.96g 1-pentaesulfonic acid sodium salt in 1-L buffered to 3.00 with Phophoric Acid

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

75

25

2

20

50

50

3

30

40

60

4

35

30

70

5

45

30

70

6

50

75

25

Flow Rate

1.20 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info