Carboxy-THC from urine using Strata-X-Drug B and Zebron Drug-1 10x0.18x0.18

GC Conditions

Column

Zebron ZB-Drug-1, GC Cap. Column 10 m x 0.18 mm x 0.18 µm, Ea

Part No

7CD-G023-08

Phase Name

ZB-Drug-1

Injection

Split 10:1 @ 280°C

Carrier Gas

Constant Flow Helium, 0.7 mL/min

Detection

Mass Selective (MSD) @ (340°C)

Detection Info

Sample Notes

Analytes are spiked into blank urine @ the SAMSHA cutoff level of 15ng/mL. After elution from SPE sorbent the sample is blown down and then derivatized with BSTFA by heating @ 70ºc for 20min.

Sample Preparation Details

Pretreatment Note

Evaporate eluant to dryness and reconstitute in 25µL ethyl acetate/50µL BSTFA with 1%TMCS. Incubate at 70ºC for 20min

Load

Load 2.50 mL Sample diluted in Buffered urine

Wash 1

2mL of 100mM sodium acetate (pH 5.0)

Wash 2

2mL of 30/70 ACN/100mM sodium acetate (pH 5.0)

Dry

15 min under full vacuum

Elution 1

2mL of 85:15 Ethyl acetate :Isopropanol

Evaporation

Evaporate to dryness

Reconstitute

75 µL 25µL ethyl acetate/50µL BSTFA with 1%TMCS