Ibuprofen by HPLC on Luna 5um C18(2) 250x4.6mm
LC Conditions
Column
Luna 5 µm C18(2) 100 Å, LC Column 250 x 4.6 mm, Ea
Brand
Luna
Part No
00G-4252-E0
Phase Name
C18(2)
Elution Type
Isocratic
Mobile Phase
A: 600:400 ACN:Water with 4g chloroacetic acid adjusted to pH 3.0 with ammonium hydroxide
Gradient Profile
Step No.
Time(min)
%A
%B
1
10
100
0
Flow Rate
2.00 mL/min
Temperature
30°C
Detection
UV-Vis Abs.-Variable Wave.(UV) @ (22°C)
Detection Info
Sample Notes
The USP Resolution requirement between any two peaks is not less than 2.5, and the tailing factor for any individual peak is not more than 2.5. On the LUNA 5um C18(2) the resolution between Ibuprofen and Valerophenone is 7.55; valerophenone and Ibuprofen related compound C is 6.11. The tailing factors for Ibuprofen, Valerophenone and Ibuprofen related compound C are 1.2, 1.45, and 1.26, respectively. The max back pressure was 215 bar.
Order Items Used in This Application
- 1. Ibuprofen
- 2. Valerophenone
- 3. Ibuprofen related compound C
-OEChem-06211003152D<br><br> 33 33 0 1 0 0 0 0 0999 V2000<br> 4.5981 3.3450 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 1.8450 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -2.6550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -2.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 -1.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 0.8450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 1.8450 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 2.8660 -3.6550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -2.1550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -0.6550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 -0.6550 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 0.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 0.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 2.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 2.3450 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -2.0350 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.9441 -2.7376 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3426 -2.0473 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 2.6950 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2460 -3.6550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 -4.2750 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4860 -3.6550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3100 -1.6181 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4631 -1.8450 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.6900 -2.6919 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3291 -0.9650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 -0.9650 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3291 0.6550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 0.6550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.1760 2.8819 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3291 2.6550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5560 1.8081 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.1350 3.6550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 15 1 0 0 0 0<br> 1 33 1 0 0 0 0<br> 2 15 2 0 0 0 0<br> 3 4 1 0 0 0 0<br> 3 8 1 0 0 0 0<br> 3 9 1 0 0 0 0<br> 3 16 1 0 0 0 0<br> 4 5 1 0 0 0 0<br> 4 17 1 0 0 0 0<br> 4 18 1 0 0 0 0<br> 5 10 2 0 0 0 0<br> 5 11 1 0 0 0 0<br> 6 7 1 0 0 0 0<br> 6 12 2 0 0 0 0<br> 6 13 1 0 0 0 0<br> 7 14 1 0 0 0 0<br> 7 15 1 0 0 0 0<br> 7 19 1 0 0 0 0<br> 8 20 1 0 0 0 0<br> 8 21 1 0 0 0 0<br> 8 22 1 0 0 0 0<br> 9 23 1 0 0 0 0<br> 9 24 1 0 0 0 0<br> 9 25 1 0 0 0 0<br> 10 12 1 0 0 0 0<br> 10 26 1 0 0 0 0<br> 11 13 2 0 0 0 0<br> 11 27 1 0 0 0 0<br> 12 28 1 0 0 0 0<br> 13 29 1 0 0 0 0<br> 14 30 1 0 0 0 0<br> 14 31 1 0 0 0 0<br> 14 32 1 0 0 0 0<br>M END
Compound Name
Ibuprofen
CID
3672
Molecular Formula
C13H18O2
Molecular Weight
206
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1
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