Polycyclic Aromatic Hydrocarbons (PAHs) in Water byZebron ZB-5MS

GC Conditions

Column

Zebron ZB-5MS, GC Cap. Column 30 m x 0.25 mm x 0.25 µm, Ea

Part No

7HG-G010-11

Phase Name

ZB-5MS

Injection

Split 15:1 @ 310°C

Carrier Gas

Constant Flow Helium, 1.4 mL/min

Detection

Mass Spectrometer (MS) @ (270°C)

Detection Info

Sample Preparation Details

Pretreatment Note

After the elution step, there will be two phases present, and organic and an aqueous. Using a pasture pipet, extract the bottom layer and transport it to a new test tube and blowdown the effluent to dryness. Each sample is carefully dried to dryness, and stopped quickly to prevent loss of more volitile PAH from evaporating. Evaporation was carried out @ 45C water bath with Nitrogen blowing down on the samples. The ISS was spiked in to the final sample right before blowdown.

Condition 1

10 mL Dichloromethane at 2 mL/min

Condition 2

10 mL Methanol at 2 mL/min

Condition 3

20 mL MilliQ Water at 2 mL/min

Load

Load 100.00 mL Sample diluted in 75:25 MilliQ Water:Acetonitrile

Wash 1

3 mL 50/50 Methanol Water at 1 mL/min

Dry

30 seconds

Elution 1

6 mL Dichloromethane at 1 mL/min

Evaporation

Evaporate to dryness

Reconstitute

100 µL Dichloromethane