Fluoroquinolones by LCMS using Kinetex XB-C18 2.6µm, 50 x 2.1 mm
LC Conditions
Column
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand
Kinetex
Part No
00B-4496-AN
Phase Name
XB-C18
Elution Type
Gradient
Mobile Phase
A: 0.1% Formic acid
B: Methanol
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
85
15
2
5
10
90
3
6
10
90
4
6
10
90
Flow Rate
0.40 mL/min
Temperature
40°C
Detection
Mass Spectrometer (MS) @ (550°C)
Detection Info
Sample Notes
Source temp: 550ºC Polarity: Postive Dwell: 25 msec
Sample Preparation Details
Description
Strata-X 33 µm Polymeric Reversed Phase, 200 mg / 6 mL, Tubes, 30/Pk
Part No
8B-S100-FCH
Condition 1
3 mL Methanol at 2 mL/min
Condition 2
3 mL Water at 2 mL/min
Load
Load 10.00 mL Sample diluted in Phosphate buffer with 2% TCA
Wash 1
2 at 2
Dry
5min @ >10 in Hg
Elution 1
2 mL Methanol/ACN/Acetic acid 49/49/2 at 1 mL/min
Evaporation
Evaporate to dryness
Reconstitute
500 µL 0.1% Formic acid
Order Items Used in This Application
- 1. Ofloxacin
- 2. Norfloxacin
- 3. Norfloxacin-d5
- 4. Ciprofloxacin
- 5. Ciprofloxacin-d8
- 6. Enrofloxacin
- 7. Difloxacin
- 8. Sparfloxacin
-OEChem-06211003152D<br><br> 46 49 0 1 0 0 0 0 0999 V2000<br> 6.3301 2.6342 0.0000 F 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5541 -0.3726 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9179 0.1458 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9644 -2.8348 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.8008 -1.3181 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 1.6342 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3462 -1.4073 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 2.6342 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 1.1342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5981 2.6342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4482 -1.9351 0.0000 C 0 0 3 0 0 0 0 0 0 0 0 0<br> 5.4641 1.1342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3301 -0.3658 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4641 0.1342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8660 1.6342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7320 3.1342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5461 -1.4142 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1962 0.1342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3301 1.6342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2233 -1.8701 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1962 1.1342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4520 -2.9350 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0556 -0.3606 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 3.1342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0690 -1.3522 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9448 -1.8350 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3335 0.6593 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.1306 0.6593 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2087 2.5265 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8101 3.2168 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.9869 -2.2420 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2554 1.7419 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.6540 1.0516 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.1306 3.6092 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.3335 3.6092 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.9360 -1.3034 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3325 -1.9963 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2431 -2.4897 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.7331 1.4442 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.8320 -2.9374 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.4544 -3.5550 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0720 -2.9327 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3100 3.6711 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4631 3.4442 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.6900 2.5973 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.5074 -3.1342 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 19 1 0 0 0 0<br> 2 14 1 0 0 0 0<br> 2 17 1 0 0 0 0<br> 3 23 2 0 0 0 0<br> 4 26 1 0 0 0 0<br> 4 46 1 0 0 0 0<br> 5 26 2 0 0 0 0<br> 6 9 1 0 0 0 0<br> 6 10 1 0 0 0 0<br> 6 12 1 0 0 0 0<br> 7 11 1 0 0 0 0<br> 7 13 1 0 0 0 0<br> 7 20 1 0 0 0 0<br> 8 15 1 0 0 0 0<br> 8 16 1 0 0 0 0<br> 8 24 1 0 0 0 0<br> 9 15 1 0 0 0 0<br> 9 27 1 0 0 0 0<br> 9 28 1 0 0 0 0<br> 10 16 1 0 0 0 0<br> 10 29 1 0 0 0 0<br> 10 30 1 0 0 0 0<br> 11 17 1 0 0 0 0<br> 11 22 1 0 0 0 0<br> 11 31 1 0 0 0 0<br> 12 14 1 0 0 0 0<br> 12 19 2 0 0 0 0<br> 13 14 2 0 0 0 0<br> 13 18 1 0 0 0 0<br> 15 32 1 0 0 0 0<br> 15 33 1 0 0 0 0<br> 16 34 1 0 0 0 0<br> 16 35 1 0 0 0 0<br> 17 36 1 0 0 0 0<br> 17 37 1 0 0 0 0<br> 18 21 2 0 0 0 0<br> 18 23 1 0 0 0 0<br> 19 21 1 0 0 0 0<br> 20 25 2 0 0 0 0<br> 20 38 1 0 0 0 0<br> 21 39 1 0 0 0 0<br> 22 40 1 0 0 0 0<br> 22 41 1 0 0 0 0<br> 22 42 1 0 0 0 0<br> 23 25 1 0 0 0 0<br> 24 43 1 0 0 0 0<br> 24 44 1 0 0 0 0<br> 24 45 1 0 0 0 0<br> 25 26 1 0 0 0 0<br>M END
Compound Name
Ofloxacin
CID
4583
Molecular Formula
C18H20FN3O4
Molecular Weight
361
No. Hydrogen Bond Acceptors
8
No. Hydrogen Bond Donors
1
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