Humic Acids using Kinetex C8 50x2.1 mm on ABI LC-MS 4000

LC Conditions

Column

Kinetex 2.6 µm C8 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4497-AN

Phase Name

C8

Elution Type

Gradient

Mobile Phase

A: 5mM Ammoniium Acetate

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

2

5

95

3

6

5

95

4

6.01

85

15

Flow Rate

0.40 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata PAH, 1.5 g / 6 mL, Tubes, 30/Pk

Part No

8B-S130-7CH

Condition 1

10 mL Dichloromethane at 5 mL/min

Condition 2

20 mL Methanol at 5 mL/min

Condition 3

20 mL MilliQ Water at 5 mL/min

Load

Load 100.00 mL Sample diluted in 75:25 MilliQ Water:Acetonitrile

Wash 1

5 mL 50:50 Methanol:MilliQ Water at 2 mL/min

Dry

15 seconds

Elution 1

6 mL Dichloromethane at 2 mL/min

Evaporation

Evaporate to dryness

Reconstitute

100 µL Dichlromethane