Benzodiazepines from urine using Strata-X-Drug N on a Kinetex C18 2.6µm, 50x2.1mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Kinetex

Part No

00B-4462-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid

B: 0.1% Formic acid in methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

65

35

2

4

5

95

3

4.01

65

35

4

7

65

35

Flow Rate

300.00 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Preparation Details

Pretreatment Note

Evaporate eluant under a stream of N2 gas at 50ºC. Reconstitute with 1mL of 35% methanol

Load

Load Sample

Wash 1

2 mLs of Acetonitrile:Water (20:80) (v:v)

Dry

10 minutes @ full vacuum

Elution 1

2mLs of Ethyl acetate:Isopropanol (85:15) (v:v)

Evaporation

Evaporate to dryness

Reconstitute

1 mL 35% methanol in water