Water soluble vitamins on Luna HILIC by LC/MS/MS

LC Conditions

Column

Luna 3 µm HILIC 200 Å, LC Column 100 x 2 mm, Ea

Brand

Luna

Part No

00D-4449-B0

Phase Name

HILIC

Elution Type

Gradient

Mobile Phase

A: 60%ACN:40% 100 mM Ammonium Formate, pH 3.2

B: 9010% 100 mM Ammonium Formate, pH 3.2

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

0

100

2

5

0

100

3

5.1

100

0

4

7

100

0

5

7.1

0

100

Flow Rate

0.50 mL/min

Temperature

22°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, HILIC 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8328 - Holder Part No.: KJ0-4282