Barbiturates in urine using Strata-X-Drug N and Kinetex C18 2.6µm 100x2.1mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 100 x 2.1 mm, Ea

Brand

Kinetex

Part No

00D-4462-AN

Phase Name

C18

Elution Type

Gradient

Gradient Profile

Step No.

Time(min)

%A

%B

2

0

90

10

3

10

55

45

4

10.01

10

90

5

12

10

90

6

12.01

90

10

7

16

90

10

Flow Rate

400.00 mL/min

Temperature

0°C

Detection

Mass Spectrometer (MS) @ (22°C)

Detection Info

Sample Preparation Details

Pretreatment Note

Evaporate eluant to dryness under stream of N2 (50ºC). Reconstitute samples with 1mL of 10% acetonitrile

Load

Load Sample

Wash 1

2 mLs of 0.1N HCl

Wash 2

2 mLs of Methanol/0.1N HCl (30:70)

Dry

10 min under full vacuum

Elution 1

2 mLs of Ethylacetate/IPA (85:15) (v:v)

Evaporation

Evaporate to dryness

Reconstitute

1 mL 10% acetonitrile