Purification of Bivalirudin (1% Loading) on Luna 10u-PREP C18(2)

LC Conditions

Column

Luna 10 µm PREP C18(2) 100 Å, LC Column 250 x 4.6 mm, Ea

Brand

Luna

Part No

00G-4324-E0

Phase Name

PREP C18(2)

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA in Water

B: 0.1% TFA in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

40

65

35

3

45

20

80

4

55

85

15

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-4287 - Holder Part No.: KJ0-4282