8 Steroid Mix on Kinetex 1.7u XB-C18 150x2.1mm using Acqutiy UPLC System

LC Conditions

Column

Kinetex 1.7 µm XB-C18 100 Å, LC Column 150 x 2.1 mm, Ea

Brand

Kinetex

Part No

00F-4498-AN

Phase Name

XB-C18

Elution Type

Gradient

Mobile Phase

A: MilliQ Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

70

30

2

5

0

100

Flow Rate

0.50 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info