Hi Temp Stability - Injection on new Aeris WIDEPORE column

LC Conditions

Column

Aeris 3.6 µm WIDEPORE XB-C18 200 Å, LC Column 50 x 2.1 mm, Ea

Brand

Aeris

Part No

00B-4482-AN

Phase Name

WIDEPORE XB-C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% TFA

B: Acetonitrile with 0.1% TFA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

3

97

3

3

23

35

65

Flow Rate

0.31 mL/min

Temperature

90°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

Application was actually run on a 50x4.6mm ID running at a flow rate of 1.5 mL/min. 50 x 4.6 mm which is not a commercially available part number. Information switched to 50x2.1mm for those that wish to replicate method.