Hi Temp Stability - Injection on Aeris WIDEPORE after 1050 cycles

LC Conditions

Column

Aeris 3.6 µm WIDEPORE XB-C18 200 Å, LC Column 50 x 2.1 mm, Ea

Brand

Aeris

Part No

00B-4482-AN

Phase Name

WIDEPORE XB-C18

Elution Type

Gradient

Mobile Phase

A: Water with 0.1% TFA

B: Acetonitrile with 0.1% TFA

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

3

97

3

3

23

35

65

Flow Rate

0.31 mL/min

Temperature

90°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

Test was run on a 50x4.6 Aeris WIDEPORE 3.6u XB-C18 run at 1.5 mL/min which is not commercially available at this time. Application was modifed to reflect the use of a 2.1 mm column for those that wish to replicat the data