Aeris Batch Reproducalbity #4: ABN7 on Aeris PEPTIDE 1.7u XB-C18 50x4.6mm

LC Conditions

Column

Aeris 1.7 µm PEPTIDE XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea

Brand

Aeris

Part No

00B-4506-AN

Phase Name

PEPTIDE XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in AcCN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

4

5

95

3

6

95

5

Flow Rate

0.38 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

Actual QC testing is done on a 50 x 4.6 mm running at 1.85 mL/min. Column is not an active PN so column was entered as a 50 x 2.1 mm running at 0.384 mL/min for those that would want to replicate the experiment on a commercially available column.