Soft Drink Additives (Coke Zero) on Kinetex 1.7u C18 100x2.10mm on Shimadzu Nexera

LC Conditions

Column

Kinetex 1.7 µm C18 100 Å, LC Column 100 x 2.1 mm, Ea

Brand

Kinetex

Part No

00D-4475-AN

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Phosphoric Acid in Water

B: 0.1% Phosphoric Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0.01

95

5

2

0.68

95

5

3

2.68

60

40

4

4.34

60

40

5

5.01

5

95

6

5.02

95

5

7

7.01

95

5

Flow Rate

0.36 mL/min

Temperature

30°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime. - SecurityGuard ULTRA Cartridges, UHPLC C18 for 4.6mm ID Columns, 3/Pk Part No.: AJ0-8768 - Holder Part No.: AJ0-9000