NSAID's in Human Urine using Kinetex 2.6 µ C8 150 x 2.10 mm on Shimadzu Nexera

LC Conditions

Column

Kinetex 2.6 µm C8 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Kinetex

Part No

00D-4497-E0

Phase Name

C8

Elution Type

Gradient

Mobile Phase

A: 0.1 Phosphoric Acid in Water

B: 0.1% Phosphoric Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

70

30

2

4

50

50

3

7.5

30

70

4

9

5

95

5

10

70

30

Flow Rate

1.50 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info