Soft Drink Additives (Coke Zero) on Kinetex 2.6u C18 100x4.60mm on Shimadzu Nexera

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Kinetex

Part No

00D-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Phosphoric Acid in Water

B: 0.1% Phosphoric Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0.01

95

5

2

0.68

95

5

3

2.68

60

40

4

4.34

60

40

5

5.01

5

95

6

5.02

95

5

7

7.01

95

5

Flow Rate

1.80 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info