Steroids Mix on Kinetex 2.6µ C18

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 50 x 4.6 mm, Ea

Brand

Kinetex

Part No

00B-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

6

40

60

Flow Rate

1.85 mL/min

Temperature

0°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info