Hydroxy Vitamin D Isomers on Synergi Polar-RP

LC Conditions

Column

Synergi 2.5 µm Polar-RP 100 Å, LC Column 100 x 2 mm, Ea

Brand

Synergi

Part No

00D-4371-B0

Phase Name

Polar-RP

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in DI H2O

B: 0.1% Formic Acid in MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

25

75

2

2

20

80

3

3.8

20

80

4

3.81

25

75

5

6

25

75

Flow Rate

400.00 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime. - SecurityGuard ULTRA Cartridges, UHPLC Phenyl for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8788 - Holder Part No.: AJ0-9000