Hydroxy Vitamin D Isomers on Kinetex 2.6um C18 30 x 3mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 30 x 3 mm, Ea

Brand

Kinetex

Part No

00A-4462-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in DI H2O

B: 0.1% Formic Acid in ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

45

55

2

0.3

20

80

3

1

20

80

4

1.1

5

95

5

1.5

5

95

6

1.51

45

55

7

2

45

55

Flow Rate

1000.00 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info