Aldosterone on Kinetex 2.6 um XB-C18 50x3 mm ID
LC Conditions
Column
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 3 mm, Ea
Brand
Kinetex
Part No
00B-4496-Y0
Phase Name
XB-C18
Elution Type
Gradient
Mobile Phase
A: DI Water
B: Methanol/Acetonitrile (50:50)
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
70
30
2
4
10
90
3
5
10
90
4
5.1
70
30
5
7
70
30
Flow Rate
500.00 mL/min
Temperature
22°C
Detection
Tandem Mass Spec (MS-MS) @ (22°C)
Detection Info
Sample Notes
SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime. - SecurityGuard ULTRA Cartridges, UHPLC C18 for 3.0mm ID Columns, 3/Pk Part No.: AJ0-8775 - Holder Part No.: AJ0-9000
Sample Preparation Details
Description
Strata-X-A 33 µm Polymeric Strong Anion, 60 mg / 3 mL, Tubes, 50/Pk
Part No
8B-S123-UBJ
Condition 1
3 mL Methanol
Condition 2
3 mL 25 mM Ammonium bicarbonate
Load
Load 1.50 mL Sample diluted in 25 mM Ammonium bicarbonate (pH 8.9-9.0)
Wash 1
3 mL 25 mM Ammonium bicarbonate
Wash 2
3 mL 25% Methanol in water
Dry
5 min at high vacuum
Elution 1
1 mL Ethyl acetate/Isopropanol/Ammonium hydroxide (7:2:1)
Elution 2
1 mL Ethyl acetate/Isopropanol/Ammonium hydroxide (7:2:1)
Evaporation
Evaporate to dryness
Reconstitute
100 µL Methanol/Water (30:70)
Order Items Used in This Application
- 1. Aldosterone
- 2. Cortisone
-OEChem-06211003152D<br><br> 54 57 0 1 0 0 0 0 0999 V2000<br> 4.7950 0.7792 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5271 1.7792 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.9821 2.2787 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -2.7732 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9391 2.6912 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3931 -0.7208 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 6.5271 -1.2208 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 7.3931 0.2792 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 5.6610 -0.7208 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 4.7510 -1.2277 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 5.6610 0.2792 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 6.5271 0.7792 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.3393 0.5839 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 8.3393 -1.0256 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5431 -2.2624 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.9229 -0.2208 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7430 -2.2693 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6451 -2.7901 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8242 -0.6636 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3931 1.2792 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7587 -0.2277 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6500 1.5344 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8763 -1.1916 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8076 -2.8190 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8680 -2.2765 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.6285 1.7406 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4830 -1.5661 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2664 -1.6402 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.3972 -0.2958 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.6610 0.8992 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9256 1.2541 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1285 1.2541 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.9018 1.0232 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0883 -1.5925 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8767 -1.3348 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.7612 -2.8427 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.1523 -2.1468 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.3838 -0.6356 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.3838 0.1939 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2478 -3.2661 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.0460 -3.2630 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2324 -0.1969 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4343 -0.1816 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.9300 1.5892 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.3787 -0.2325 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7635 0.3923 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.1388 -0.2229 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.6718 -0.6063 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2647 -1.2929 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.8100 -3.4390 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.7950 1.3992 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.6490 1.1210 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.2423 1.6533 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 10.5458 2.8190 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 11 1 1 1 0 0 0<br> 1 51 1 0 0 0 0<br> 2 20 2 0 0 0 0<br> 3 22 2 0 0 0 0<br> 4 25 2 0 0 0 0<br> 5 26 1 0 0 0 0<br> 5 54 1 0 0 0 0<br> 6 7 1 0 0 0 0<br> 6 8 1 0 0 0 0<br> 6 14 1 0 0 0 0<br> 6 27 1 6 0 0 0<br> 7 9 1 0 0 0 0<br> 7 15 1 0 0 0 0<br> 7 28 1 1 0 0 0<br> 8 12 1 0 0 0 0<br> 8 13 1 0 0 0 0<br> 8 20 1 1 0 0 0<br> 9 10 1 0 0 0 0<br> 9 11 1 0 0 0 0<br> 9 29 1 6 0 0 0<br> 10 17 1 0 0 0 0<br> 10 19 1 0 0 0 0<br> 10 21 1 1 0 0 0<br> 11 12 1 0 0 0 0<br> 11 30 1 0 0 0 0<br> 12 31 1 0 0 0 0<br> 12 32 1 0 0 0 0<br> 13 16 1 0 0 0 0<br> 13 22 1 1 0 0 0<br> 13 33 1 0 0 0 0<br> 14 16 1 0 0 0 0<br> 14 34 1 0 0 0 0<br> 14 35 1 0 0 0 0<br> 15 18 1 0 0 0 0<br> 15 36 1 0 0 0 0<br> 15 37 1 0 0 0 0<br> 16 38 1 0 0 0 0<br> 16 39 1 0 0 0 0<br> 17 18 1 0 0 0 0<br> 17 24 2 0 0 0 0<br> 18 40 1 0 0 0 0<br> 18 41 1 0 0 0 0<br> 19 23 1 0 0 0 0<br> 19 42 1 0 0 0 0<br> 19 43 1 0 0 0 0<br> 20 44 1 0 0 0 0<br> 21 45 1 0 0 0 0<br> 21 46 1 0 0 0 0<br> 21 47 1 0 0 0 0<br> 22 26 1 0 0 0 0<br> 23 25 1 0 0 0 0<br> 23 48 1 0 0 0 0<br> 23 49 1 0 0 0 0<br> 24 25 1 0 0 0 0<br> 24 50 1 0 0 0 0<br> 26 52 1 0 0 0 0<br> 26 53 1 0 0 0 0<br>M END
Compound Name
Aldosterone
CID
5839
Molecular Formula
C21H28O5
Molecular Weight
360
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
2
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