Quantification of Carbamates Fungicides in Beverage by LC/MS/MS

LC Conditions

Column

Gemini 3 µm C18 110 Å, LC Column 50 x 2 mm, Ea

Brand

Gemini

Part No

00B-4439-B0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 5mM ammonium formate

B: 5mM ammonium formate in acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

2.5

5

95

3

4

5

95

4

4.01

85

15

5

8

85

15

Flow Rate

0.50 mL/min

Temperature

28°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S100-TBJ

Condition 1

1 mL Methanol

Condition 2

1 mL 25 mM Ammonium acetete pH 5.5

Load

Load 1.40 mL Sample diluted in pretreated sample

Wash 1

1 mL 25 mM Ammonium acetete pH 5.5

Wash 2

1 mL Methanol/Water (15:85)

Dry

2 min at 10mm Hg

Elution 1

0.5 mL 5% Formic acid in Methanol

Evaporation

Evaporate to dryness

Reconstitute

1 mL Initial mobile phase