Penicillins in Meat by LC/MS/MS

LC Conditions

Column

Gemini 3 µm C18 110 Å, LC Column 150 x 4.6 mm, Ea

Brand

Gemini

Part No

00F-4439-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Ammonia in water

B: 95% Acetronitrile, 5% water and 0.1% ammonia

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

98

2

2

2

98

2

3

2.42

90

10

4

6

50

50

5

6.1

0

100

6

6.6

0

100

7

6.8

98

2

8

8

98

2

Flow Rate

0.80 mL/min

Temperature

40°C

Detection

Tandem Mass Spec (MS-MS) @ (600°C)

Detection Info

Sample Notes

This separation uses IMethod Kit Part Number KH0-9047 SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 3.0mm ID, 10/Pk Part No.: AJ0-7597 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata C18-E (55 µm, 70 Å), 200 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S001-FBJ

Pretreatment Note

The equilibration time at conditon 0 was 4 minutes before each injection.

Condition 1

4 mL Methanol at 30 µL/min

Condition 2

4 mL Water at 30 µL/min

Load

Load 2.00 mL Sample diluted in Filtrate

Wash 1

4 mL 0.1% Formic Acid in water

Elution 1

2 mL Acetonitrile at 30 µL/min

Elution 2

2 mL Acetonitrile at 30 µL/min

Evaporation

Evaporate to dryness

Reconstitute

200 µL Water