Separation of Benzodiazepines

LC Conditions

Column

Synergi 4 µm Hydro-RP 80 Å, LC Column 50 x 2 mm, Ea

Brand

Synergi

Part No

00B-4375-B0

Phase Name

Hydro-RP

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid in DI H2O

B: 0.1% formic Acid in ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0.5

90

10

2

0.7

80

20

3

5.9

25

75

4

6

5

95

5

6.2

5

95

6

6.3

90

10

7

8.5

90

10

Flow Rate

500.00 mL/min

Temperature

40°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, AQ C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7510 - Holder Part No.: KJ0-4282