Pharma ABN Test 15 Mix on Optimized Agilent 1100 system using Kinetex 2.6u C18 100x4.6mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 100 x 4.6 mm, Ea

Brand

Kinetex

Part No

00D-4462-E0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in Water

B: 0.1% Formic Acid in Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

95

5

2

0.2

95

5

3

5

5

95

4

6

5

95

5

6.1

95

5

Flow Rate

1.50 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime. - SecurityGuard ULTRA Cartridges, UHPLC C18 for 4.6mm ID Columns, 3/Pk Part No.: AJ0-8768 - Holder Part No.: AJ0-9000