Underivatized MMA and Succinic Acid in Human Urine on Gemini C18, 3u, 100x3 mm

LC Conditions

Column

Gemini 3 µm C18 110 Å, LC Column 100 x 3 mm, Ea

Brand

Gemini

Part No

00D-4439-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in DI H2O

B: 0.1% Formic Acid + 10 mM Ammonium Formate in MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

1.5

5

95

3

2.5

5

95

4

2.51

85

15

5

4.5

85

15

Flow Rate

700.00 mL/min

Temperature

40°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282

Sample Preparation Details

Description

Strata-X-AW 33 µm Polymeric Weak Anion, 30 mg / 1 mL, Tubes, 100/Pk

Part No

8B-S038-TAK

Pretreatment Note

This method is for the analysis of underivatized MMA.

Condition 1

1 mL Methanol

Condition 2

1 mL 25 mM Ammonium Formate

Load

Load Sample

Wash 1

0.5 mL Methanol/Water (50:50)

Dry

Dry under high vacuum for 5-10 min

Elution 1

0.6 mL Ammonium hydroxide/Methanol (2:98)

Elution 2

0.6 mL Ammonium hydroxide/Methanol (2:98)

Evaporation

Evaporate to dryness

Reconstitute

200 µL 0.1% Formic acid