MMA Calibration from Human Serum on Gemini C18, 3u, 100x3 mm

LC Conditions

Column

Gemini 3 µm C18 110 Å, LC Column 100 x 3 mm, Ea

Brand

Gemini

Part No

00D-4439-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic Acid in DI H2O

B: 0.1% Formic Acid + 10 mM Ammonium Formate in MeOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

85

15

2

1.5

5

95

3

2.5

5

95

4

2.51

85

15

5

4.5

85

15

Flow Rate

700.00 mL/min

Temperature

40°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Gemini C18 4 x 2.0mm ID, 10/Pk Part No.: AJ0-7596 - Holder Part No.: KJ0-4282