Extraction and Analysis of Metronidazole in Facial Cleansers
LC Conditions
Column
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
Brand
Kinetex
Part No
00B-4496-AN
Phase Name
XB-C18
Elution Type
Gradient
Mobile Phase
A: 0.1% Formic Acid
B: MeOH
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
95
5
2
2
60
40
3
3
60
40
4
3.1
95
5
5
5
95
5
Flow Rate
300.00 mL/min
Temperature
25°C
Detection
Mass Spectrometer (MS) @ (650°C)
Detection Info
Sample Notes
SecurityGuard™ ULTRA Guard Cartridge System extends column lifetime. - SecurityGuard ULTRA Cartridges, UHPLC C18 for 2.1mm ID Columns, 3/Pk Part No.: AJ0-8782 - Holder Part No.: AJ0-9000
Sample Preparation Details
Description
Strata-X-C 33 µm Polymeric Strong Cation, 30 mg / 3 mL, Tubes, 50/Pk
Part No
8B-S029-TBJ
Pretreatment Note
Evaporate to dryness at 50 C. Reconstitute in 200 uL of 10:90 MeOH:0.1% Formic Acid
Condition 1
1 mL of MeOH
Condition 2
1 mL of 0.1N HCl
Load
Load Sample
Wash 1
3 mL of 0.1N HCl
Wash 2
3 mL of MeOH
Wash 3
6 mL of Ethyl Acetate
Dry
5 min under full vacuum
Elution 1
1 mL of 5:95 NH4OH:MeOH (v:v)
Evaporation
Evaporate to dryness
Reconstitute
200 µL 10:90 MeOH:0.1% Formic Acid
Order Items Used in This Application
- 1. Metronidazole
- 2. 13-C2 15-N-Metronidazole
-OEChem-06211003152D<br><br> 21 21 0 0 0 0 0 0 0999 V2000<br> 2.8940 -1.8644 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.2633 1.5835 0.0000 O 0 5 0 0 0 0 0 0 0 0 0 0<br> 5.7281 -0.0638 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.7601 0.6356 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.2601 2.1744 0.0000 N 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5202 0.9143 0.0000 N 0 3 0 0 0 0 0 0 0 0 0 0<br> 3.7601 -0.3644 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.9511 1.2234 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.5691 1.2234 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8940 -0.8644 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.2601 2.1744 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 0.9143 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.9721 -0.9470 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3707 -0.2568 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.6820 -0.2818 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.2835 -0.9721 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.6245 2.6760 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.8084 1.5040 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1.4103 0.7228 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.1916 0.3247 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.3571 -2.1744 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 10 1 0 0 0 0<br> 1 21 1 0 0 0 0<br> 2 6 1 0 0 0 0<br> 3 6 2 0 0 0 0<br> 4 7 1 0 0 0 0<br> 4 8 1 0 0 0 0<br> 4 9 1 0 0 0 0<br> 5 8 2 0 0 0 0<br> 5 11 1 0 0 0 0<br> 6 9 1 0 0 0 0<br> 7 10 1 0 0 0 0<br> 7 13 1 0 0 0 0<br> 7 14 1 0 0 0 0<br> 8 12 1 0 0 0 0<br> 9 11 2 0 0 0 0<br> 10 15 1 0 0 0 0<br> 10 16 1 0 0 0 0<br> 11 17 1 0 0 0 0<br> 12 18 1 0 0 0 0<br> 12 19 1 0 0 0 0<br> 12 20 1 0 0 0 0<br>M CHG 2 2 -1 6 1<br>M END
Compound Name
Metronidazole
CID
4173
Molecular Formula
C6H9N3O3
Molecular Weight
171
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
1
Similar Applications
Reversed Phase
Extraction and Analysis of Metronidazole in Facial Cleansers
Kinetex 2.6 µm XB-C18 100 Å, LC Column 50 x 2.1 mm, Ea
20631