Histamine by LCMS using Luna 3um HILIC 100x2.1mm

LC Conditions

Column

Luna 3 µm HILIC 200 Å, LC Column 100 x 2 mm, Ea

Brand

Luna

Part No

00D-4449-B0

Phase Name

HILIC

Elution Type

Gradient

Mobile Phase

A: 50:45:5 ACN:DI H2O: 100 mM Amm. Form, pH 3.22

B: 95:5 ACN:100 mM Amm. Formate, pH 3.22

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

17

83

2

1.5

100

0

3

2.5

100

0

4

2.6

17

83

5

4.5

17

83

Flow Rate

0.60 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X-CW 33 µm Polymeric Weak Cation, 30 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S035-TBJ

Condition 1

1 mL Methanol

Condition 2

1 mL DI water

Load

Load Sample

Wash 1

2 mL DI water

Wash 2

2 mL MeOH

Dry

2 min at high vacuum level, ~20-30 in Hg

Elution 1

0.5 mL 87:13 B:A, fortified to contain 5% formic acid

Evaporation

Evaporate to dryness

Reconstitute

0 µL initial mobile phase