Estradiol and estrone analysis by LCMS using Gemini 3um NX-C18 50x2mm
LC Conditions
Column
Gemini 3 µm NX-C18 110 Å, LC Column 50 x 2 mm, Ea
Brand
Gemini
Part No
00B-4453-B0
Phase Name
NX-C18
Elution Type
Gradient
Mobile Phase
A: H2O + 0.5 mM ammonium hydroxide
B: MeOH + 0.5 mM ammonium hydroxide
Gradient Profile
Step No.
Time(min)
%A
%B
1
0
90
10
2
0.5
90
10
3
2.5
20
80
4
5
10
90
5
5.2
90
10
6
6
90
10
Flow Rate
0.55 mL/min
Temperature
22°C
Detection
Tandem Mass Spec (MS-MS) @ (22°C)
Detection Info
Order Items Used in This Application
- 1. Estrone
- 2. Estrone-D4
- 3. Estradiol
- 4. Estradiol-D3
- 5. Estriol
- 6. Estriol-D3
-OEChem-06211003152D<br><br> 42 45 0 1 0 0 0 0 0999 V2000<br> 9.1857 2.1767 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.5357 -2.1309 0.0000 O 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.9288 -0.0785 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.9288 0.9215 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.0628 -0.5785 0.0000 C 0 0 1 0 0 0 0 0 0 0 0 0<br> 6.1968 -0.0785 0.0000 C 0 0 2 0 0 0 0 0 0 0 0 0<br> 7.0628 1.4215 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8750 -0.3833 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1968 0.9215 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.0789 -1.6200 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.8750 1.2262 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.4586 0.4215 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.9288 1.9215 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2868 -0.5854 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.1808 -2.1478 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.2787 -1.6270 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3599 -0.0213 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3433 -2.1767 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4120 -0.5493 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 3.4037 -1.6342 0.0000 C 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.0188 -0.9238 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.8022 -0.9979 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.9329 0.3465 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.4613 1.8964 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.6643 1.8964 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.6240 -0.9502 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.4124 -0.6925 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.9847 1.5041 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.5862 0.8138 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.2969 -2.2004 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.6880 -1.5046 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9195 0.0067 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 9.9195 0.8362 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 8.5488 1.9215 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.9288 2.5415 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 7.3088 1.9215 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 5.7835 -2.6238 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 6.5818 -2.6207 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3719 0.5986 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 4.3457 -2.7967 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.8787 -0.2331 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 2.0000 -1.8188 0.0000 H 0 0 0 0 0 0 0 0 0 0 0 0<br> 1 11 2 0 0 0 0<br> 2 20 1 0 0 0 0<br> 2 42 1 0 0 0 0<br> 3 4 1 0 0 0 0<br> 3 5 1 0 0 0 0<br> 3 8 1 0 0 0 0<br> 3 21 1 6 0 0 0<br> 4 7 1 0 0 0 0<br> 4 11 1 0 0 0 0<br> 4 13 1 1 0 0 0<br> 5 6 1 0 0 0 0<br> 5 10 1 0 0 0 0<br> 5 22 1 1 0 0 0<br> 6 9 1 0 0 0 0<br> 6 14 1 0 0 0 0<br> 6 23 1 6 0 0 0<br> 7 9 1 0 0 0 0<br> 7 24 1 0 0 0 0<br> 7 25 1 0 0 0 0<br> 8 12 1 0 0 0 0<br> 8 26 1 0 0 0 0<br> 8 27 1 0 0 0 0<br> 9 28 1 0 0 0 0<br> 9 29 1 0 0 0 0<br> 10 15 1 0 0 0 0<br> 10 30 1 0 0 0 0<br> 10 31 1 0 0 0 0<br> 11 12 1 0 0 0 0<br> 12 32 1 0 0 0 0<br> 12 33 1 0 0 0 0<br> 13 34 1 0 0 0 0<br> 13 35 1 0 0 0 0<br> 13 36 1 0 0 0 0<br> 14 16 2 0 0 0 0<br> 14 17 1 0 0 0 0<br> 15 16 1 0 0 0 0<br> 15 37 1 0 0 0 0<br> 15 38 1 0 0 0 0<br> 16 18 1 0 0 0 0<br> 17 19 2 0 0 0 0<br> 17 39 1 0 0 0 0<br> 18 20 2 0 0 0 0<br> 18 40 1 0 0 0 0<br> 19 20 1 0 0 0 0<br> 19 41 1 0 0 0 0<br>M END
Compound Name
Estrone
CID
5870
Molecular Formula
C18H22O2
Molecular Weight
270
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1
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