Steroid panel by LCMS using Kinetex 2.6u C18 100x3.0mm

LC Conditions

Column

Kinetex 2.6 µm C18 100 Å, LC Column 100 x 3 mm, Ea

Brand

Kinetex

Part No

00D-4462-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: Water

B: Methanol

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

2

90

10

3

3

60

40

4

7

30

70

5

10

20

80

6

10.2

5

95

7

13

5

95

8

13.2

90

10

9

16

90

10

Flow Rate

0.55 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info