Figure 7. K-Casein Tryptic Digest on Aeris Peptide 1.7 µm XB-C18 & Aquity 1.7 µm BEH C18

LC Conditions

Column

Aeris 1.7 µm PEPTIDE XB-C18 100 Å, LC Column 100 x 2.1 mm, Ea

Brand

Aeris

Part No

00D-4506-AN

Phase Name

PEPTIDE XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% Formic acid +10 mM NH4 formate

B: 0.1% Formic acid +10 mM NH4 formate in ACN

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

1

97

3

3

20

50

50

4

22

5

95

5

22.1

97

3

Flow Rate

0.40 mL/min

Temperature

50°C

Detection

Mass Spectrometer (MS) @ (550°C)

Detection Info