Figure 5. Method Optimization – BSA Tryptic Digest with Different Flow Rates

LC Conditions

Column

Aeris 3.6 µm PEPTIDE XB-C18 100 Å, LC Column 150 x 2.1 mm, Ea

Brand

Aeris

Part No

00F-4507-AN

Phase Name

PEPTIDE XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% FA+10mM NH4 formate

B: 0.1% FA+10mM NH4 formate in ACN/Water (95:5)

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

97

3

2

3

97

3

3

50

55

45

4

52

5

95

5

52.1

97

3

Flow Rate

0.50 mL/min

Temperature

40°C

Detection

Mass Spectrometer (MS) @ (550°C)

Detection Info