Degraded Myoglobin on Aeris WIDEPORE 3.6u XB-C18 vs. Jup 5u C18 overlay

LC Conditions

Column

Aeris 3.6 µm WIDEPORE XB-C18 200 Å, LC Column 150 x 2.1 mm, Ea

Brand

Aeris

Part No

00F-4482-AN

Phase Name

WIDEPORE XB-C18

Elution Type

Gradient

Mobile Phase

A: 0.1% TFA

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

90

10

2

5

90

10

3

50

50

50

Flow Rate

0.20 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Diode Array (PDA) @ (22°C)

Detection Info

Sample Notes

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard ULTRA Cartridges, UHPLC WIDEPORE C18 for 2.1mm ID, 3/Pk Part No.: AJ0-8783 - Holder Part No.: AJ0-9000