Biogenic Amines by LCMS using Kinetex 1.7u C18 50x3.0mm

LC Conditions

Column

Kinetex 1.7 µm C18 100 Å, LC Column 50 x 3 mm, Ea

Brand

Kinetex

Part No

00B-4475-Y0

Phase Name

C18

Elution Type

Gradient

Mobile Phase

A: 30mM Ammonium formate

B: Acetonitrile

Gradient Profile

Step No.

Time(min)

%A

%B

1

0

80

20

2

3

40

60

3

3.1

0

100

4

4

0

100

5

4.1

80

20

6

5

80

20

Flow Rate

600.00 mL/min

Temperature

22°C

Detection

Tandem Mass Spec (MS-MS) @ (22°C)

Detection Info

Sample Preparation Details

Description

Strata-X-C 33 µm Polymeric Strong Cation, 60 mg / 3 mL, Tubes, 50/Pk

Part No

8B-S029-UBJ

Condition 1

3 mL Methanol at 1 mL/min

Condition 2

4 mL Water

Load

Load Sample

Wash 1

3 mL 0.1M HCl

Wash 2

3 mL Methanol

Elution 1

3 mL 0.2M Ammonia Solution made up in methanol

Evaporation

Evaporate to dryness

Reconstitute

0 µL Mobile phase