The Effect of Mobile Phase Additive on the Separation of PPIs in NP Elution Mode

LC Conditions

Column

Lux 5 µm Cellulose-1, LC Column 250 x 4.6 mm, Ea

Brand

Lux

Part No

00G-4459-E0

Phase Name

Cellulose-1

Elution Type

Isocratic

Mobile Phase

A: 0.1% FA in Hexane

B: 0.1% FA in IPA or EtOH

Gradient Profile

Step No.

Time(min)

%A

%B

1

20

60

40

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info