The Effect of Mobile Phase Additive on the Separation of PPIs in RP Elution Mode-1

LC Conditions

Column

Lux 5 µm Cellulose-2, LC Column 250 x 4.6 mm, Ea

Brand

Lux

Part No

00G-4457-E0

Phase Name

Cellulose-2

Elution Type

Isocratic

Mobile Phase

A: 0.1% FA in CH3CN

B: 0.1%FA in water

Gradient Profile

Step No.

Time(min)

%A

%B

1

10

60

40

Flow Rate

1.00 mL/min

Temperature

22°C

Detection

UV-Vis Abs.-Variable Wave.(UV) @ (22°C)

Detection Info